WaferGen Bio-systems, Inc.

Apollo 324

Products

Apollo 324™

The Apollo 324™ Next Generation Sample Preparation System provides Clinical Researchers with the greatest sample preparation flexibility, workflow convenience, and system reliability.

Apollo 324™ Advantages

Flexible

  • Run 1 -96 samples in one day in any batch size

Convenient

  • Walk away capability, as little as 30 minutes of hands on time

Reliable

  • Completely enclosed system minimizes contamination

Apollo 324™ NGS Library Prep System

Prepare libraries in as little as one hour.
The Apollo 324 is a benchtop platform that provides a walk away solution to the tedium of library preparation. Select your protocol, load up your reagents, press RUN. Walk away completely. No need to batch your samples, prepare any number at any time.

Highlights

This system provides you with the most powerful sample preparation across multiple sample sizes and protocols. In this recording, we highlight the Apollo 324's features, including all of the pre-installed and validated sample preparation protocols.

The Apollo 324™ System Touchscreen Interface ?>

The Apollo 324™ System Touchscreen Interface

The Apollo 324 System’s easy-to-use touchscreen walks you through each protocol setup. Color-coded deck layouts on the screen match our color-coded PrepX™ reagent tubes, so even inexperienced operators can set up runs without errors. Our low throughput protocols take less than 15 minutes to set up and our high throughput protocols take only 30 minutes to set up. When everything’s ready, the system will display a green RUN button. Setup, press RUN and walk away completely.

Resources

Automated VeriSeq™ Library Preparation

In Vitro Fertilization (IVF) efforts paired with preimplantation genetic screening (PGS) methods increase favorable pregnancy outcomes by identifying aneuploidy in embryos and excluding them from implantation.

The VeriSeq PGS kit screens all 24 chromosomes for aneuploidy in a single next-generation sequencing assay. Manual VeriSeq library preparation is time consuming, laborious and prone to human error. Automating VeriSeq library preparation on the Apollo 324 Library Prep System increases reliability, reproducibility and efficiency of the VeriSeq library prep workflow, allowing researchers to conduct other experiments while the Apollo 324 completes library preparation.

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Automated VeriSeq™ Library Preparation

Automated Illumina Library Preparation from 0.5 ng ChIP DNA

The Application Note “Automated Library Preparation from 0.5 ng of ChIP DNA” describes Illumina library preparation of chromatin immunoprecipitation (ChIP) sample DNA input with the Apollo 324™ System at the Wellcome Trust Sanger Institute.

Data for the Note were contributed by Michael Quail PhD of the Wellcome Trust Sanger Institute.

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Automated Illumina Library Preparation from 0.5 ng ChIP DNA

Recommendations For DNA Input, Using Adapters, Optimizing PCR, and Loading Sample Libraries

This technical note presents instructions for adjusting experimental conditions when using the Apollo 324™ System to produce DNA fragment libraries with custom molecular weight range (size in base pairs) profiles.

The binding of DNA with magnetic beads used by the Apollo 324 System depends, in part, on the concentrations of polyethylene glycol (PEG) and salt (NaCl) in the DNA solution, and the lengths of individual fragments. User modifications to the PEG and buffer concentrations used by the Apollo 324 System selects the size range of the library produced. By making relatively straightforward modifications to the PEG and salt concentrations of BeadX™ tubes used by the Apollo 324 System during fragment library cleanup and size selection, researchers can extend the laboratory utility of the system beyond standard next-generation sequencing library preparation to automate the production of fragment libraries compatible with ChIP (Chromatin Immunoprecipitation), RNA, and de novo sequencing applications. This document presents buffer conditions and buffer modification instructions for producing libraries compatible with each of these four applications.

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Recommendations For DNA Input, Using Adapters, Optimizing PCR, and Loading Sample Libraries

DNA Fragment Library Size Range Selection

This technical note presents recommendations for fragmented DNA input, using adapters, and optimizing the PCR reaction when using the Apollo 324™ System, and loading prepared sample libraries on a sequencer.

Download:
DNA Fragment Library Size Range Selection

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